ZENG Haiao, WU Jinglu. A METHOD TO EXTRACT AMMONIUM AND NITRATE FROM FRESHWATER FOR NITROGEN ISOTOPE ANALYSIS[J]. Marine Geology & Quaternary Geology, 2013, 33(6): 173-177. DOI: 10.3724/SP.J.1140.2013.06173
Citation: ZENG Haiao, WU Jinglu. A METHOD TO EXTRACT AMMONIUM AND NITRATE FROM FRESHWATER FOR NITROGEN ISOTOPE ANALYSIS[J]. Marine Geology & Quaternary Geology, 2013, 33(6): 173-177. DOI: 10.3724/SP.J.1140.2013.06173
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A METHOD TO EXTRACT AMMONIUM AND NITRATE FROM FRESHWATER FOR NITROGEN ISOTOPE ANALYSIS

  • State Key Laboratory of Lake Science and Environment, Nanjing Institute of Geography and Limnology, Chinese Academy of Sciences, Nanjing 210008, China

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  • Received Date: April 18, 2012
  • Revised Date: July 10, 2012
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    • Abstract
  • This paper presents a simple and rapid method to concentrate ammonium and nitrate from freshwater samples for an on-line isotope analysis using an elemental analyzer coupled to a mass spectrometer. Ammonium and nitrate are collected by passing the water-sample through pre-filled, disposable, ion exchanging resin columns. The ammonium and nitrate in sample solutions are separated as ammonium into diluted H2SO4 by sequential distillation techniques. Then, the excrescent SO42- in the distillate is precipitated directly as an insoluble salt of BaSO4. (NH4)2SO4 is collected by freezing-drying filtrate, which is subsequently combusted in an elemental analyzer for δ15N determination. The sorption efficiency of 001×7 cationic resin and 732 cationic resin for 2 L sample solutions with ammonium and nitrate concentration of 0.5, 1.0, 5.0 mg/L is >95.1%, elution recovery is >95%, showing insignificant isotope fractionation during precipitation. Analyses of the solutions prepared from (NH4)2SO4 and KNO3 distributed, and also the rain, river, lake and well samples showed that the method can produce good quality of isotope measurements.
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    • References (13)
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